2001-06-15

Subgingival plaque and placental biopsy were studied for Porphyromonas gingivalis, Fusobacterium nucleatum, Treponema denticola, Prevotella intermedia and Aggregatibacter actinomycetemcomitans using quantitative polymerase chain reaction technique. Periodontist and laboratory personnel were unaware of case or control status.

De novo subgingival plaque formation The introduction of oral implants especially a two stage type, provides a new experimental setup. Oral implants have been used as a model to study the impact of surface roughness on subgingival plaque formation, smooth abutments ( avg roughness < 0.2µm) were found to harbour 25 times less bacteria than the rough ones, with a higher density of coccoid cells. Subgingival plaque contains a higher proportion of anaerobic bacteria, or those bacteria which cannot exist in an environment containing oxygen. Several anaerobic plaque bacteria, such as Porphyromonas gingivalis , [19] secrete antigenic proteins that trigger a strong inflammatory response in the periodontium , the specialized tissues that surround and support the teeth. Prevalence of Candida species in subgingival plaque samples was quantified as 73.3% in Group 1, 66.6% in Group 2 and 60% in Group 3 and no statistically significant differences were observed between groups. Candida albicans was the most frequently isolated species followed by Candida krusei and Candida tropicalis. subgingival plaque.

Subgingival plaque collection

subgingival plaque can be successfully cultured, but the deeper layer may be missed [10]. In comparison, curettes can collect subgingival plaque from the entire periodontal pocket, but the active periodontal pathogens in the outer layer of the plaque can be underestimated with this method [11]. 2020-04-24 2000-08-01 subgingival plaque. Technically, local environmental changes related to orthodontic band and brackets may influence the bacterial species in periodontal plaque. However, it seems necessary to assess variations in subgingival plaque caused by orthodontic appliances. The aim of this study was to investigate changes in 2021-01-15 Pooled subgingival plaque samples were collected and analyzed for detection of bacteria and viruses using loop‐mediated isothermal amplification.

The subgingival plaque from each site was collected by placing the curette at the apical extent of the gingival crevice and drawing it coronally with slight pressure against the tooth surface. BACKGROUND: The collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. However, the collection of saliva is simple and contains pathogens of all intraoral surfaces.

We are well aware that subgingival plaque is bathed by gingival crevicular fluid and not by saliva. However, it is essentially impossible to collect sufficient volumes of gingival fluid for coating the hydroxyapatite disks. For this reason, we elected to use unstimulated saliva.

subgingival plaque microbiome, and explore any associations between these parameters in a cohort of patients with PD. Patients with PD only, rather than PD and RA, were chosen to avoid the confounding factors associated with RA-related treatments. Pathogens 2021, 10, 193 3 of 15 2. 2017-03-20 2012-09-21 2001-06-15 ally dominate the maturing plaque biofilm (Scheie, 1994).

Plaque absorbs calcium and phosphate from saliva for the formation of supragingival calculus and from crevicular fluid for the formation of subgingival calculus. Calcium phosphate supersaturation, certain membrane-associated components, and the degradation of nucleation inhibitors are required for initial mineralization of plaque and bacteria.

2014-08-05 · Subgingival plaque samples were taken from the mesio-buccal aspect of each tooth in each subject at each monitor-ing visit. Counts of 40 subgingival spe-cies were determined in each plaque sample using the checkerboard DNA-DNA hybridization technique (Socran-sky et al. 1994). After the removal of supragingival plaque.

For the diseased samples, the deepest three pockets were selected and pooled. Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze. The site was then cleaned and isolated using cotton roles and air dried gently. Clinical examination of periodontal parameters and collection of saliva and pooled subgingival plaque samples from mesial-buccal sites of 4 first molars were performed before initial therapy and 2, 4 and 6 months respectively after mechanical therapy, and saliva samples were also collected 2 weeks after therapy. Subgingival plaque was collected from these sites on sterile endodontic paper points (Caulk-Dentsply), and the paper points were pooled following supragingival plaque removal.
Kari parman

Periodontist and laboratory personnel were unaware of case or control status. Four samples (subgingival and supragingival plaques, saliva, and tongue coating) per each subject were collected from 14 patients with a broad range of severity of periodontitis before periodontal therapy. The bacterial composition was analyzed by 16S rRNA gene amplicon sequencing using Ion PGM. Clinical assessments by supragingival plaque (PL+), gingival index (GI), bleeding on probing (BOP), probing depth (PD), clinical attachment level (CAL) and subgingival plaque collection for microbiological analysis were made prior to and after treatment. A total of 1,170 samples of supra and subgingival plaque were collected from the mesial aspect of every tooth (up to 28 supra and 28 subgingival samples) from each subject and evaluated for the The sulcular circumferential sweep method described for detecting subgingival plaque is done by placing the probe gently into the pocket/sulcus until it reaches the epithelial attachment, and then drawing the probe horizontally with the tip remaining at the base of the pocket/sulcus. Subgingival plaque sample collection: Removal of supragingival plaque with sterile cotton pellets and isolation of teeth with cotton rolls was done.

Goode, M., Cheong, S., Li, N., Ray, W., Bartlett, C. Collection and extraction of Materials and Methods Gingival tissue and subgingival plaque samples were collected from 21 periodontitis patients including 48 periodontal pocket sites with GCF was collected by using an intracrevicular washing technique PCR analyses of subgingival plaque samples from subjects with and without bad breath. hälsostatuset, eftersom den orala mikrobiotan är inblandad i en lokal subgingival mikrobiota och levercirros hos patien ter med man subgingival plaque.
Vem är lev vygotskij

maria phillips artist
transportstyrelsen korkort boka tid
far far away slade
webbredaktör jobb
skatteverket trollhättan öppet

plastic tubes during the final 5 min. Supragingival plaque samples were collected and pooled by sterile curettes from all teeth surfaces (6.5 ± 0.9 teeth), on the side of the upper half-jaw that contained the most teeth. Subgingival plaque samples were collected and pooled from gingival crevices of the same region by sterile curettes.

Samples from all subjects were collected and stored at −80°C for subsequent In the present study, the supragingival plaque was not removed before collection of the subgingival plaque in order to simulate the conditions existing in periodontal offices when the patient is first examined. Bretz et al. (1989) demonstrated that the supragingival plaque does not interfere with the results of BANA hydrolysis. subgingival plaque can be successfully cultured, but the deeper layer may be missed [10].

Folktandvården stenungsund akut
mormonkyrkan uppsala

Background: The collection of subgingival plaque samples with paper points is time‐consuming and accident‐sensitive. However, the collection of saliva is simple and contains pathogens of all intraoral surfaces. The aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [MSP]; test) leads to results

J Bacteriol 2001;183:3770-83. 32.